Introduction: Carney Complex is an autosomal dominant syndrome that is defined with different tumors including myxoma in different organs, endocrine tumors and lentiginosis lesions. This is the first case report of this syndrome from Iran.Case: The patient is a 27 year old girl, referred with flank pain. Physical examination revealed hirsutism, truncal obesity, hyperpigmantasion and hypertension; Cushing's syndrome was suggested and confirmed with related classic biochemical tests. She had history of cardiac myxoma during her childhood and had been operated twice.Pituitary microadenoma and right adrenal adenoma were reported on MRI and CT-scan, respectively. Initially laparascopic right adrenalectomy was done; as expected, no remission in signs of Cushing's syndrome was observed after surgery. By laparoscopic adrenalectomy of the other site, Cushing's syndrome resolved. Pathologic report of 1st operation was adrenal adenoma with surrounding pigmented micronodular hyperplasia and of the 2nd one was just pigmented micronodular hyperplasia.Conclusion: We have reported a patient with Carney syndrome along with Cushing's syndrome due to bilateral adrenal hyperplasia and an adenoma in contralateral adrenal and microadenoma of the pituitary as an incidentaloma. This is a new presentation of Carney syndrome.

There has been considerable interest in developing albumin nanoparticles as drug delivery devices. Albumin is an important endogenous antioxidant due to its potential of acting as reactive oxygen species scavenger. On the other hand, toxicity of silver nanoparticles had been demonstrated on cancer cell lines. In the present study, Ag NPs coated with BSA NPs were synthesized by silver nanoparticles which were coated with bovine serum albumin (BSA) via desolvation technique. The Ag NPs coated with BSA NPs formation was confirmed by UV-Vis spectroscopy and Dynamic Light Scattering (DLS). Human breast cancer cells (MCF7 cells) were then cultured in the presence of the nanoparticles to evaluate the cytotoxicity of Ag NPs coated with BSA NPs by the MTT colorimetric technique. The antioxidant activities of Ag NPs coated with BSA NPs were evaluated in terms of their inhibition of autoxidation rate of pyrogallol as superoxide. The effect of Ag NPs coated with BSA NPs on MCF7 exhibit a dose-dependent toxicity for the cell tested and the viability of MCF-7 decreased to 50% (LD50) at the concentration of 5 mg/mL. The IC50 value of antioxidant activities of Ag NPs coated with BSA NPs were 8 mg/mL which demonstrated that Ag NPs coated with BSA NPs were good superoxide scavengers. In conclusion, our data show that Ag NPs coated with BSA NPs had antioxidant and anticancer activities in MCF-7 cells.

Background and purpose: Simultaneous exposure of nanoparticles can cause serious damage to aquatic organisms including fish. The aim of this study was to investigate the toxicity effect of subchronic exposure of copper oxide nanoparticles (CuO NPs) in presence of titanium dioxide nanoparticles (TiO2 NPs) on the histopathology of gill and kidney tissues of fish model in vivo exposure.Materials and methods: In this study, one non-lethal concentration of TiO2 NPs, two nonlethal concentrations of CuO NPs, two concentrations of TiO2 NPs and CuO NPs mixture, and a control group (no chemicals) were used. After 10 days of exposure to these materials, samples of gill and kidney tissues were collected. In order to study classical histology the samples were prepared by hematoxylineosin staining and the slides were photographed by optical microscopy.Results: The damages caused in gill were as following: secondary curved blades, increased mucus secretion, increase in the agglomeration of secondary lamellae, and aneurism and hyperplasia.Also, blood vessels dilation, vacuoles, necrosis, focal tubule degeneration, and increase in melanomacrophage were observed in kidney tissue of common carp.Conclusion: The study suggested that toxic effects of CuO NPs on tissues of common carp in the joint presence of TiO2 NPs were synergistic.

Introduction: Polychaeta, or bristle worms, are a class of marine Annelid worms with segmented bodies. Each segment has paired fleshy appendages called parapodia, equipped with bristles known as chaetae made of chitin. Over 10,000 species are described, and polychaetes play vital ecological roles as predators and prey in their ecosystems. Polychaeta, Hediste diversicolor is one of the important live foods in fish and crustacean nutrition such as sturgeon and shrimp. This study aimed to investigate the effect of different levels of selenium nanoparticles (Se-NPs) and iron nanoparticles (Fe-NPs) on the growth and survival of H. diversicolor.Materials and methods: 48000 Hediste worms (with an average wet weight of 37 mg) were distributed in 12 tanks (50-L volume) as a completely randomized design with four treatments and three replications (4000 worms per m2). Treatments included control (without adding nanoparticles), T1 = 50 mg/kg Fe-NPs + 0.5 mg/kg Se-NPs, T2 = 100 mg/kg Fe-NPs + 1 mg/kg Se-NPs and T3 = Fe-NPs 200 mg/kg + 2 mg/kg Se-NPs. Before starting the experiment, Hediste worms were adapted to the tank conditions for one week, and then they were fed diets containing selenium and iron nanoparticles for 12 weeks.Results and discussion: The results indicated that increasing the amounts of selenium nanoparticles (Se-NPs) and iron nanoparticles (Fe-NPs) in the diet significantly enhanced the biomass and survival rate of Hediste worms. Specifically, as the concentrations of selenium and iron in the treatments increased, the length of L3 (the combined length of the first three segments: prostomium, peristomium, and first chaetiger) decreased significantly. T2 and T3 exhibited shorter L3 lengths compared to the control group. The highest survival rates were observed in T2 (65.5%) and T3 (66.6%), while the greatest biomass was recorded in T3 (1514.8 g/m²). Notably, Hediste worms in the control group consumed significantly more food and displayed the highest food conversion ratio (FCR). In contrast, the lowest FCR values of 0.57 and 0.54 were recorded in T2 and T3, respectively, indicating a positive effect of these nanoparticles on improving FCR.Conclusion: The combined application of Se-NPs at a concentration of 2 mg/kg with 200 mg/kg Fe-NPs demonstrated a superior effect on the growth and survival rate of Hediste diversicolor compared to both the control diet and other treatments.Conflicts of interest: Authors have no conflict of interest to declare for the publication of the present work.Acknowledgments: This research was conducted as a study opportunity for members of the faculty of the Agricultural Research, Education, and Extension Organization in Society and Industry. We would like to thank the Zist Palayeshgar Khazar Company (ZPK) and the International Sturgeon Research Institute.

Introduction: Men with unexplained infertility and azoospermia are often observed in the context of genetic defects. The expression of a wide variety of genes is developmentally regulated  during human meiosis. Synaptonemal Protein 3 (SYCP3) gene, located on chromosome 12, encodes a DNA-binding protein as the structural component of the synaptonemal Complex,which mediates the synopsis or homologous pairing of chromosomes during meiosis. Absence of SYCP3 in mice may lead to male infertility as well as female sub-fertility. SYCP3 expression analysis could be a tool for the prediction of human spermatogenesis progression, especially in infertile men.Materials & Methods: SYCP3 mRNA expression in testicular samples of 110 patients with non-obstructive azoospermia were studied in Avesina Infertility Clinic in Tehran, Iran during 2005 and early 2006. Semi-quantitative nested reverse transcriptase-PCR was employed in order to find the strength of gene expression. Using histopathological scoring for all samples, the expression level of SYCP3 during spermatogenesis was also evaluated.Results: Testicular SYCP3 mRNA expression was observed in 67 patients (60.9%). The expression level correlated with the degree of spermatogenic failure (p<0.0001). While this gene had been expressed in patients with hypo-spermatogenesis and maturation arrest, a lack of expression was seen in those with spermatogonial arrest, Sertoli cell-only syndrome and testicular atrophy.Conclusion: These data indicate that SYCP3 is expressed in the human testis and it is restricted to germ cells. Our findings, in association with those obtained in experimental animals, show that lack of SYCP3 expression may have negative effects on spermatogenesis and male fertility. SYCP3 gene expression may help detect specific spermatogenesis stages in conjunction with histopathological findings.

Two spotted spider mite (Tetranychus urticae Complex) is an important pest of  egg-plant in Iran. In the present study, population of eggs and active stages and amount of losses of the spider mite were determined on upper and lower leaves of nine egg-plant cultivars in Varamin during 2001-2002. The experiment was in a randomized complete block design with three replications. Each plot consisted of four rows and on each row ten egg-plants were cultivated. Spider mite population on all cultivars was checked at weekly samplings, from July to October. Number of mites (eggs and active stages) on upper and lower leaves were then counted by stereo binocular microscope. Loss percent of mite was assessed by measuring the weight of ten randomly selected fruits of each cultivar in three replications at fifteen day intervals during fruiting period. Analysis of variance on data showed significant differences of mite population on upper and lower leaves of different cultivars and also among the cultivars. In 2000, the highest mean populations of active mites on lower leaves, 37.80 and 46.87, and on upper leaves, 27.19 and 38.08 were recorded on Borazjan cv. in naturally and artificially infested treatments, respectively. In 2001, egg and active mite populations on cultivar were significantly different only in artificially infested treatment. The maximum numbers of eggs (63.47) and active stage (107.94) were recorded on Imami 905 cultivar. Maximum and minimum losses of 69.42% and 12.86% were recorded on Imami 905 and Ghalami Varamin cultivars. These cultivars were determined as the most susceptible and resistant cultivars to mite damage, respectively.

Background and purpose: In recent years, new psychoactive substances have become a serious challenge for countries in the field of combating the production, sale, prevention and treatment of addicts and victims of these substances. Therefore, the present research was conducted with the aim of investigating the methods of scientific identification and diagnosis of new non-herbal psychoactive substances (NPs).Method: The current research is practical in terms of its purpose and experimental in terms of its execution method. The statistical population of the research was samples of exposed substances from foreign travelers in the period of 1395-1398, which were identified and diagnosed using machine analysis methods. The basis of identification and diagnosis was matching the reported spectra of the device with the spectra available in the UNODC reference. The study of the library and documentary part of the research was also based on a systematic review in the period from 1980 to 2022 and included all the articles published in the electronic database with the combination of appropriate keywords.Findings: In this research, sample preparation and device settings were done in mass spectrometry and infrared spectrometry and then the prepared samples were injected into the corresponding devices. In mass spectrometry of ion fragments with higher intensity and in infrared spectrometry, the numbers of functional groups were obtained, and these spectra were interpreted in the conclusion.Conclusion: The spectra obtained from the mass spectrometer and infrared spectrometer were interpreted and after comparing the reported spectra of the device with the spectra available in the UNODC reference, 8 types of new psychoactive substances were identified. They contained different amounts of addictive substances. These compounds are: 1-Dimethoxybromoamphetamine, 2-Ethyldimethoxyphenylethanamide, 3-Fluoro-MDMB-Pinaka, 4-Fluoro-ADB-Pinaka, 5-Fluoro-MDMB-Botanika and 6-MDMB-EN-Pinaka.